

Description
xHieff NGS™ FFPE DNA Repair Reagent is specifically designed for FFPE samples to repair varying degrees of damage in low-quality FFPE DNA, enhancing library yield and sequencing quality. This product can be directly used with Yeasen Hieff NGS™ Library Preparation Kits (Cat#12197/Cat# 12927) and the A-tailing module (Cat# 12608), with compatible systems that can repair 5 ng to 1 μg of fragmented FFPE DNA. Additionally, this product is also suitable for use with Yeasen Fragmentation Enzyme-based Library Preparation Kit.
Features
High Repair Efficiency: Provides higher repair efficiency for FFPE DNA.
Streamlined Workflow: Simple operation with strong adaptability.
High Compatibility: Enzyme components can be used with the end-repair and A-tailing system of library preparation kits, requiring only a single buffer to complete FFPE DNA repair and end-repair/dA-tailing.
Applications
Repair of damaged low-quality FFPE samples
Library preparation for low-quality FFPE samples
Specifications
|
Cat.No. |
12606ES24/ 12606ES96 |
|
Size |
24 T /96 T |
Components
|
Components No. |
Name |
12606ES24 |
12606ES96 |
|
12606-A |
FFPE DNA Repair Mix |
96 μL |
384 μL |
|
12606-B |
Endprep Buffer 2.0 |
144 μL |
576 μL |
Shipping and Storage
This product should be stored at -25~-15℃ for 1 year.
Figures

Figure1. The impact of FFPE repair reagents on library yield
During the library construction process, two sets of experiments were conducted to compare the same samples. One group included a repair reagent in the end-repair and A-tailing step, while the other group did not. The experimental results showed that the FFPE repair reagent significantly increased library yield for low-quality FFPE samples by repairing the samples, whereas its effect on high-quality FFPE samples was less pronounced.
Documents:
Safety Data Sheet
Manuals
12606_Manual_Ver.EN20260202.pdf
